Conclusions: This systematic review infers that urologists can safely use either option of fragmentation and basket extraction or dusting without extraction to achieve similar outcomes as both ...

This process of DNA extraction—collecting samples, lysing tissue and purifying or isolating target DNA—is the most time and labour-intensive step of most DNA tests and typically …

Herein, we review the two primary laser treatment approaches: dusting and fragmentation with extraction. Methods: We reviewed PubMed and MEDLINE databases from January 1976 through January 2017. ... Humphreys M, Knudsen B, Krambeck A et al. Ureteroscopic Laser Lithotripsy: A Review of Dusting vs Fragmentation with Extraction. Journal of ...

This detailed fragmentation information enables disambiguation between multiple compositional possibilities for glycopeptide structure. Among the protein extraction and glycopeptide enrichment methods compared, EtOH precipitation and HILIC-SPE most efficiently extracted proteins in human milk and enriched tryptic N-glycopeptides, …

INTRODUCTION. Sperm DNA fragmentation (SDF) refers to single-stranded or double-stranded breaks in the genome of spermatozoa. Because the mature male gamete lacks the ability to repair DNA damage [], these breaks tend to persist and can negatively influence male reproductive potential and outcomes.Three primary mechanisms can lead to SDF: …

Satellite data and modelling reveal that tropical forest fragments have similar size distributions across continents, and that forest fragmentation is close to a critical point, beyond which ...

Systematic examination of protein extraction, proteolytic glycopeptide enrichment and MS/MS fragmentation techniques for site-specific profiling of human milk N-glycoproteins ... D.C. Dallas et al. ... (Kim & Dallas, 2021). A study compared several different fragmentation modes of endogenous neuropeptides and characterized the peptides …

Forest fragmentation is a major driver of global biodiversity loss and ecosystem degradation 1,2,3,4.Identifying which areas have the most severe forest fragmentation is a fundamental task in ...

Comparison and outcomes of dusting versus stone fragmentation and extraction in retrograde intrarenal surgery: results of a systematic review and meta-analysis ... Pietropaolo et al. have shown how high-power Holmium lasers have brought to the endourology armamentarium the ability to select high-frequency laser pulses while …

This systematic review infers that urologists can safely use either option of fragmentation and basket extraction or dusting without extraction to achieve similar outcomes as both techniques are similar for efficacy and safety.

sécurité du travail et, en général, l'efficacité des travaux à ciel ouvert. Dans la plupart des cas. la préparation des roches à l'extraction repose sur la destruction du massif des roches jusqu'à l'obtention de morceaux de dimensions néces­ saires et admissibles pour le travail normal de tout le complexe d'extraction et de transport.

Liu X, Li P, Widlak P et al. DFF40 induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998; 95 :8461–6. Article CAS PubMed PubMed Central Google Scholar

Mitochondria contain their own genomes that, unlike nuclear genomes, are inherited only in the maternal line. Owing to a high mutation rate and low levels of recombination of …

Esteves et al. found testicular sperm to have a three to five times lower proportion of DNA fragmentation than ejaculated sperm. In a recent prospective comparative study on the use of testicular sperm for ICSI in 172 patients with high levels of sperm DNA damage, it was found that the proportion of testicular sperm DNA damage (8.3%) was five ...

Unlike most short-read sequencing techniques, which only require very small amounts of DNA, the extraction of inhibitor-free, high molecular weight DNA, which is suitable for long-read sequencing, has always been a challenge due to …

The number and proportion of people living in urban areas are increasing rapidly (Table 19.2).Between 1970 and 2000, there was an increase in urban land area of 5.8 million ha globally (Seto et al., 2011).Between 2000 and 2010, 2.8 million ha was urbanized in China alone (World Bank Group, 2015).The terms urbanization and urban areas are not …

Stone fragmentation and basket extraction is suggested to have the advantage of a better SFR . In the present study, the SFR of Group F (78.6%) was significantly better than Group D (58.6%). ... In a randomised multicentre study conducted by Humphrey et al., similar results were reported for SFR (74.3% for fragmentation and basketting vs 58.2% ...

Fragmentation effects propagate through the whole ecosystem. (A to C) For each fragmentation treatment [reduced area in BDFFP, Wog Wog, Kansas (A); increased isolation in SRS and Moss (B); and increased edge in all experiments (C)], we summarize major findings for ecological processes at all levels of ecological organization. Each dot ...

Consideration of this non-random DNA fragmentation may allow one to unravel what factors and to what extent influence the non-uniform coverage of various genomic regions. ... Hansen et al. 24 ...

An overview of the workflow and variables tested in this study are shown in Fig. 1.We evaluated MS/MS results acquired from EThcD-fragmentation technique through comparison of HCD- and ETD-derived MS/MS spectra, the efficiency of human milk protein extraction using EtOH precipitation, TCA precipitation and filtration using the 10-kDa …

2.1. Fragmentation definitions. Habitat fragmentation can be considered as a product of non-uniform habitat loss along habitat boundaries, meaning that habitat loss and fragmentation are concurrent in the majority of non-experimental scenarios [].Precisely altered experimental landscapes are required to disentangle these innately linked …

Among the fragmentation-based approaches, Snyder et al. (2016) ... Cell-free plasma DNA extraction, sequencing, and data preprocessing.

In fragmented DNA, PCR-based methods quantify the number of intact regions at a specific amplicon length. However, the relationship between the population of DNA fragments within a sample and the ...

Introduction. Forest fragmentation is a major driver of global biodiversity loss and ecosystem degradation 1 – 4.Identifying which areas have the most severe forest fragmentation is a fundamental task in ecology, but the findings from investigations into the patterns of global forest fragmentation have been inconsistent 5 – 7.Forests in tropical …

Tous les malades avaient bénéficié d'une méatotomie endoscopique horizontale avec fragmentation et extraction des calculs. Le temps opératoire moyen a été de 27 mn (18-58).

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Mitochondria contain their own genomes that, unlike nuclear genomes, are inherited only in the maternal line. Owing to a high mutation rate and low levels of recombination of mitrochondrial DNA ...

Johnston, A.D., Lu, J., Korbie, D. et al. Modelling clinical DNA fragmentation in the development of universal PCR-based assays for bisulfite-converted, formalin-fixed and …

Here, we quantify and map global patterns of habitat fragmentation for the world's terrestrial mammals. We used high-resolution habitat-suitability models developed for mammals (), including 4,018 species across 26 taxonomic Orders.For each species we quantified the degree of fragmentation of high-suitability habitat (i.e., preferred habitat …

Oocytes and early embryos have been shown to repair sperm DNA damage, so the effect of sperm DNA fragmentation depends on the combined effects of sperm chromatin …

The Shokere et al. group suggested that the concentration of DNA increased slightly with increasing fragmentation of DNA. However, our results show that DNA quantification based on A 260 is not significantly affected by the …

cause of fragmentation (Allentoft . et al., 2012). By adjusting for this baseline of degradation other, more situational, factors for degradation such as the influence of …

Banks-Leite et al. (2020) conclude that habitat fragmentation or loss is contributing to the loss of thousands of species globally. Substantial studies have reported that LULC change is the major ...